Antimicrobial susceptibility profile of human pathogenic bacteria isolated from raw milk
DOI:
https://doi.org/10.14295/2238-6416.v74i3.743Keywords:
milk microbiology, food quality, resistant bacteria.Abstract
The presence of bacteria in raw milk associated with the possible indiscriminate use of antimicrobials in dairy cattle can influence the emergence of resistant bacteria. The antimicrobial susceptibility profile of nine pathogenic bacterial strains isolated from raw milk from the State of Maranhão (Acinetobacter septicus, Hafnia alvei, Lactococcus garvieae, Staphylococcus epidermidis, Pantoea spp. and Klebsiella spp.) was evaluated in this study employing the test of disc diffusion antibiogram in Müller Hinton Agar, and for the interpretation of the sensitivity categories, it was used the criteria previously established. The least efficient antimicrobial was lincomycin, in which 83.33% of the strains showed resistance. Gentamicin and tetracycline were 100% efficient against the isolates of this study. Klebsiella spp. were resistant to more than 50% of the antimicrobials tested. However, multi-resistance was observed in 50% of the isolates: Hafnia alvei, Pantoea spp. and Klebsiella spp. The verified results demonstrate the multiresistance of pathogens of great importance for public health, and the raw milk as a possible source of delivery of these multi-resistant microorganisms.
References
ALMEIDA, L. P. et al. Antibiotic residues in milk of rural properties of Uberlândia – MG region. Bioscience Journal, v. 19, n. 3, p. 83-87, 2003.
BAUER A.W. et al. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, v. 45, n. 4, p. 493-496, 1966. DOI: 10.1093/ajcp/45.4_ts.493.
BICUDO, E. L. et al. Nosocomial outbreak of Pantoea agglomerans in a pediatric urgent care center. Brazilian Journal of Infectious Diseases, v. 11, n. 2, p. 281-284, 2007. DOI: 10.1590/S1413-86702007000200023.
CANTAS, L. et al. A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota. Frontiers in Microbiology, v. 4, n. 96, p. 1-14, 2013. DOI: 10.3389/fmicb.2013.00096.
CLSI (Clinical and Laboratory Standards Institute). Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Fifth Informational Supplement. CLSI Document M100-S25. Wayne: Clinical and Laboratory Standards Institute. 2015. Disponível em: https://www.facm.ucl.ac.be/intranet/CLSI/CLSI-2015-M100-S25.unlocked.pdf. Acesso em: 07 set 2018.
CLSI (Clinical and Laboratory Standards Institute). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. 2012. Disponível em: http://antimicrobianos.com.ar/ATB/wp-content/uploads/2012/11/03-CLSI-M07-A9-2012.pdf. Acesso em: 14 jul. 2018.
ESTRELA, T. S. Resistência antimicrobiana: enfoque multilateral e resposta brasileira. In: BRASIL, MINISTÉRIO DA SAÚDE, ASSESSORIA DE ASSUNTOS INTERNACIONAIS DE SAÚDE. Saúde e política externa: os 20 anos da Assessoria de Assuntos Internacionais de Saúde (1998-2018). Brasília: Ministério da Saúde, 2018. p .307-327.
FRACALANZZA, S. A. P. Identificação, resistência a antimicrobianos e caracterização molecular de Enterococcus isolados de alimentos. 2007. 177 f. Tese (Doutorado em Vigilância Sanitária) - Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, 2007.
FREITAS, M. F. L. et al. Perfil de sensibilidade antimicrobiana in vitro de Staphylococcus coagulase positivos isolados de leite de vacas com mastite no agreste do Estado de Pernambuco. Arquivo do Instituto Biológico, v. 72, n. 2, p. 171-177, 2005.
FOOD AND DRUG ADMINISTRATION (FDA). New Drugs at FDA: CDER’s new molecular entities and new therapeutic biological products. Disponível em: http://wayback.archive-it.org/7993/20170111002417/http://www.fda.gov/Drugs/DevelopmentApprovalProcess/DrugInnovation/default.htm Acesso em: 31 jul. 2019.
GURUNG, M. et al. Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea. Journal of Dairy Science, v. 96, n. 4, p. 1997-2002, 2013.
JANDA, J. M.; ABBOTT, S. L. The Genus Hafnia: from Soup to Nuts. Clinical Microbiology Reviews, v. 19, n. 1, p. 12-28, 2006. DOI: 10.1128/cmr.19.1.12-28.2006.
KILIC, A. et al. Acinetobacter septicus sp. nov. Association with a nosocomial outbreak of bacteremia in a neonatal intensive care unit. Journal of Clinical Microbiology, v. 46, n. 3, p. 902-908, 2008. DOI: 10.1128/jcm.01876-07
LIMA, J. B. A. Caracterização genética de bactérias proteolíticas e lipolíticas autóctones do leite cru no Maranhão-Brasil. 2016. 100 f. Tese (Doutorado em Ciência Animal) – Universidade Estadual de Londrina, Londrina, 2016.
MAGIORAKOS, A. P. et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, v. 18, n.3, p. 268-281. DOI:10.1111/j.1469-0691.2011.03570.x
OLIVEIRA, C. B. S. Frequência e perfil de resistência de Klebsiella spp. em um hospital universitário de Natal/RN durante 10 anos. Jornal Brasileiro de Patologia e Medicina Laboratorial, v. 47, n. 6, p. 589-594, 2011. DOI: 10.1590/S1676-24442011000600003.
OVERDEVEST, I. et al. Extended-spectrum β-Lactamase genes of Escherichia coli in chicken meat and humans, the Netherlands. Emerging Infectious Diseases, v. 17, n. 7, p. 1216-1222, 2011.
PERNA, T. D. G. S. Prevalência de infecção hospitalar pela bactéria do gênero Klebsiella
em uma Unidade de Terapia Intensiva. Revista da Sociedade Brasileira de Clínica, v. 13, n. 2, p. 119-123, 2015.
PIESSENS, V. et al. Intra-species diversity and epidemiology varies among coagulase-negative Staphylococcus species causing bovine intramammary infections. Veterinary Microbiology, v. 155, n. 1, p. 62-71, 2012. DOI:10.1016/j.vetmic.2011.08.005
PIERI, F. A. et al. Risky consumption habits and safety of fluid milk available in retail sales outlets in Viçosa, Minas Gerais State, Brazil. Foodborne Pathogens and Disease, v. 11, n. 6, p. 490-496, 2014.
POSSUELO, L. G.; RENNER, J. D. P. Caracterização e identificação microbiológica
de Kruyvera sp. e Pantoea sp. Journal of Infection Control, v. 4, n. 2, p. 52-53, 2015.
ROCHA, C.; REYNOLDS, N. D.; SIMONS, M. P. Resistencia emergente a los antibióticos: una amenaza global y un problema crítico en el cuidado de la salud. Revista Peruana de Medicina Experimental y Salud Pública, v. 32, n. 1, p. 139-145, 2015.
SALABERRY, S. R. S. et al. Análise microbiológica e perfil de sensibilidade do Staphylococcus spp. em mastite subclínica de caprinos leiteiros. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 68, n. 2, p. 336-344, 2016.
SANTOS, N. Q. A resistência bacteriana no contexto da infecção hospitalar. Texto Contexto Enfermagem, v. 13, p. 64-70, 2004.
SILVA, L. C. C.; FAGNANI, R. Resíduos químicos no leite. In: BELOTI, V. et al. Leite: obtenção, inspeção e qualidade. Londrina: Editora Planta, 2015.
TANWAR J. et al. Multidrug resistance: An emerging crisis. Interdisciplinary Perspectives on Infectious Diseases, n. 541340, p.1-7, 2014. DOI: 10.1155/2014/541340.
TOMAZI, T. et al. Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 52, n. 5, p. 1658-1663, 2014. DOI:10.1128/jcm.03032-13.
VENDRELL, D. et al. Lactococcus garvieae in fish: A review. Comparative Immunology, Microbiology and Infectious Diseases, v. 29, n. 4, p. 177-198, 2006. DOI:10.1016/j.cimid.2006.06.003.
WILBRING, M. et al. Lactococcus garvieae causing zoonotic prosthetic valve endocarditis. Clinical Research in Cardiology, v. 100, n. 6, p. 545-546, 2011. DOI:10.1007/s00392-011-0286-3
Downloads
Published
Issue
Section
License
Term of Responsibility and Transference of Author Rights
The undersigned authors of the article entitled "INSERT TITLE" declare to have read and approved all the manuscript. The authors agree to submit it to the Journal of Candido Tostes Dairy Institute for evaluation and possible publication as true, authentic, and original results. This statement implies that the manuscript, regardless of language, was not submitted to other journals with the same purpose. The authors acknowledge that will be required to provide retractions or corrections of errors related to the article, if necessary. The authors are aware of the guidelines and editorial policy of the Journal and assign copyright to the Journal of Candido Tostes Dairy Institute with the exclusive right to print, publish and sell the product worldwide, in all languages and media. Therefore, it is forbidden to authors reproduce wholly or partially the submitted manuscript in any other part or media, print or electronic. Thus, the authors declare that accepts to transfer the rights of graphic reproduction to the Journal of Candido Tostes Dairy Institute in the case of the article with the above title (or title that later come to be adopted, to meet the suggestions of the editors and reviewers) will be published in the Journal of Candido Tostes Dairy Institute. In addition, the authors agree to appoint _(name)___________ as the corresponding author.
This term should be signed by all authors in the order of authorship, stating:
Date;
Authors name without abbreviation;
Document number;
Title/position of each author;
e-mail; and
the area for evaluation of the paper.
