Psychrotrophic and thermoduric psychrotrophic microorganisms enumeration in milk: comparison of methods
DOI:
https://doi.org/10.14295/2238-6416.v70i1.360Keywords:
temperature-resistant bacteria, methodology, thermotolerant, pasteurizationAbstract
The aim of the present study was to evaluate whether the binomial temperature/time of 21 ºC for 25 hours, already validated for enumeration of psychrotrophics in milk, presents viability for enumeration of thermoduric psychrotrophic, comparing counts of psychrotrophic and psychrotrophic thermoduric obtained from each binomial of time and temperature of incubation. Twenty-eight refrigerated raw milk
samples collected in tank trucks in the reception platform of dairy industries in the northern of Paraná were evaluated. Psychrotrophic and thermoduric psychrotrophic were enumerated at 7 °C for 10 days and at 21 °C for 25 hours. For psychrotrophic, it was observed that there was no significant difference of the counts comparing the two methods of incubation (p = 0.46), the correlation coefficient between methods was 0.90 and the linear regression considered satisfactory. For thermoduric psychrotrophic it was not observed significant difference (p = 0.17) in the counts, however, the correlation coefficient between the two methods was 0.69 and the linear regression unsatisfactory. It was also observed greater homogeneity in the distribution of the psychrotrophic counts results towards thermoduric psychrotrophic. The results demonstrate that the numeration of thermoduric psychrotrophic microorganisms by the faster methodology can change the result of the counts, since the coefficient of correlation and linear regression methods are low.
Downloads
Published
Issue
Section
License
Term of Responsibility and Transference of Author Rights
The undersigned authors of the article entitled "INSERT TITLE" declare to have read and approved all the manuscript. The authors agree to submit it to the Journal of Candido Tostes Dairy Institute for evaluation and possible publication as true, authentic, and original results. This statement implies that the manuscript, regardless of language, was not submitted to other journals with the same purpose. The authors acknowledge that will be required to provide retractions or corrections of errors related to the article, if necessary. The authors are aware of the guidelines and editorial policy of the Journal and assign copyright to the Journal of Candido Tostes Dairy Institute with the exclusive right to print, publish and sell the product worldwide, in all languages and media. Therefore, it is forbidden to authors reproduce wholly or partially the submitted manuscript in any other part or media, print or electronic. Thus, the authors declare that accepts to transfer the rights of graphic reproduction to the Journal of Candido Tostes Dairy Institute in the case of the article with the above title (or title that later come to be adopted, to meet the suggestions of the editors and reviewers) will be published in the Journal of Candido Tostes Dairy Institute. In addition, the authors agree to appoint _(name)___________ as the corresponding author.
This term should be signed by all authors in the order of authorship, stating:
Date;
Authors name without abbreviation;
Document number;
Title/position of each author;
e-mail; and
the area for evaluation of the paper.
